NXTGNT
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About NXTGNT
Why work with us?
Core facility for next generation sequencing
(epi-)genome, transcriptome, ChIP
Proven solutions
Efficient, automated workflows
NXTGNT was founded in 2008 to create a rich and unique substrate for groundbreaking research, development and valorization at Ghent University. The main goal of NXTGNT is the establishment of a reference center and core facilty for next generation sequencing (NGS), (epi-)genome and transcriptome analysis at Ghent University. NXTGNT established a unique service platform by the integration of a state-of-the-art NGS library preparation facility with several sequencing platforms and a strong expertise in -omics data-analysis (bioinformatics).
Youtube video about NXTGNT:
Sequencing the Unsequenceable, Overcoming Obstacles with Expertise
Experience
Broad range of Applications
Innovative workflows
All organisms / species
Bioinformatics
From RNA / DNA to results
NXTGNT is supported by several Ghent University partners from different faculties:
Expertise and services
Genomics
Using massively parallel sequencing the genome sequence can be accurately determined. Depending on your setting and interest the whole genome can be sequenced (even starting from a very limited amount of DNA) or specific genes or regions (e.g. exome sequencing) can be targeted.
Transcriptomics
Transcriptomics (or expression profiling) is the study of the complete set of RNA transcripts that are produced by specific cells or tissues under specific circumstances. Messenger RNAs (mRNA), micro RNAs (miRNA), long non-coding RNAs (lncRNA), etc can be sequenced and quantified, allowing differential gene expression analysis.
Epigenomics
Epigenetics research seeks to describe dynamic alterations in the transcriptional potential of a cell. The main epigenetic mechanisms are DNA methylation, histone modification, DNA accessibility and non-coding RNA-associated gene silencing. NXTGNT has all the tools in house to analyse these epigenetic mechanism in an integrated analysis.
Bioinformatics
NXTGNT can help you with your sequencing data analysis with a team of high-skilled experts. We perform data analysis starting from raw sequencing reads, working towards publication-ready results: Statistics, visualization, PCA analysis, Gene-Set Enrichment Analysis, pathway analysis, etc. Feel free to contact us regarding any question.
10X Genomics
single cell sequencing
We provide all single cell and linked-reads sequencing solutions offered by the 10X Genomics Chromium System & GemCode Technology
Pharmacogenetics
Molecular insights, connecting physicians and their patients to the latest treatment approaches through pharmacogenetics, making precision medicine a reality.
Nanopore sequencing
Oxford nanopore sequences unamplified DNA and RNA, eliminating the need for amplification steps (and the resulting quantitative and qualitative amplification bias). Long reads up to 100 kbp with direct detection of 5-methylcytosines in DNA and N6-methyladenines in RNA provide tremendous advantages in de novo assembly, detection of large structural variants, RNA isomforms, etc.
Laser-capture microdissection (LCM)
Our PALM MicroBeam is designed to give you advanced laser microdissection capabilities for subsequent DNA, and RNA analysis — whether from archive material or live cells. Applicable to cryosections, and FFPE tissue, even from standard glass slides.
Low input samples
NXTGNT is specialized in low input samples such as single cell samples, FFPE samples, and LCM samples
Meet the team
Filip Van Nieuwerburgh
Steering Committee
Prof. Filip Van Nieuwerburgh
(Ph.D. Pharm.D.)
Filip Van Nieuwerburgh heads the UGent Next Generation Sequencing core facility NXTGNT, focusing on transcriptomics, (epi)genomics and bioinformatics. He coordinated the initiative to bring together key experts to create the Expertise Centre for Sequencing
Dieter Deforce
Steering Committee
Prof. Dieter Deforce (Ph.D. Pharm.D.)
Dieter Deforce is the head of the Laboratory for Pharmaceutical Biotechnology in the Faculty of Pharmaceutical Sciences. He is the director of ProGenTomics offering proteomics and genomics services.
Wim Van Criekinge
Steering Committee
Prof. Wim Van Criekinge (Ph.D.)
Wim Van Criekinge heads Biobix, a Computational Genomics & Bioinformatics laboratory at Ghent University. Wim Van Criekinge is CSO of MDxHealth, a listed biotechnology company active in cancer diagnostics.
Daisy Flamez
Steering Committee
Daisy Flamez
Passionate towards innovation in applied biomarker research, business development and contract management, network and partnering with industrial partners, patent portfolio management, facilitation of obtaining (inter)national innovation funds, licensing, and setup of spin-offs.
Sarah De Keulenaer
Sequencing Operational Team
Sarah De Keulenaer
Project coordination. Sample preparation expert. Financial administration.
Sylvie Decraene
Sequencing Operational Team
Sylvie Decraene
Project coordination. Sample preparation expert. Financial administration.
Ellen De Meester
Sequencing Operational Team
Ellen De Meester
Project coordination. Sample preparation expert. Financial administration.
Yannick Gansemans
Bioinformatics Team
Yannick Gansemans
A biologist, a chemist, and a statistician are out hunting. The biologist shoots at a deer and misses five feet to the left. The chemist takes a shot and misses five feet to the right. The statistician yells “We got ’em!”
Sonja Skevin
Bioinformatics Team
Sonja Skevin
FASTA than the others
Guillaume Cattebeke
Bioinformatics Team
Guillaume Cattebeke
If you torture data enough it will confess.
Anne-Sofie Vermeersch
Bioinformatics Team
Anne-Sofie Vermeersch
Bring data! I’ll provide the results.
BioBix
Bioinformatics & Computational Genomics
Biobix
focuses on development of innovative data-analytical methodologies to solve complex biological questions, related to imprinting, CpG mutability, epitranscriptomics, telomere biology, micropeptide biology, cancer, aging studies, virus discovery and ecology.
Contact
Address
NXTGNT
Ghent University,
Faculty of Pharmaceutical Sciences
Ottergemsesteenweg 460
B-9000 Gent
Belgium
Inside the building
When entering the Ghent University Faculty of Pharmaceutical Sciences through the main entrance, elevators will be located in front of you. NXTGNT is situated on the third floor. When exiting the elevators, NXTGNT is located at your right-hand side.
Contact us
nxtgnt@UGent.be
Call: +32 9 264 80 59