Equipment 

State-of-the-art sample preparation

NXTGNT possesses state-of-the-art sample preparation equipment that allows to do a lot of different sample preparations in an automated, efficient and accurate way.

 

Diagenode IP-Star

Diagenode IP-Star

The Diagenode IP-Star is a compact automated system that provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. It allows for high-throughput sample preparation compatible with popular downstream applications such as qPCR and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purifications.

 

Covaris S2

Covaris S2

The Covaris S2 focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing for numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. The computer controlled device allows for shearing at a certain, set length.

 

 

10X Genomics Chromium Controller 

10X Genomics Chromium Controller

The Chromium Controller has been designed to rapidly and efficiently automate highly parallel sample partitioning and molecular barcoding. The Chromium Controller allows a user to run any Chromium Solution, spanning from genome to single cell analysis.

 

State-of-the-art High-throughput library preparation and quality control

NXTGNT is fully equipped with the latest technologies to process a high throughput of samples with the most diverse library preparation protocols. A ZEISS PALM MicroBeam Axio Observer 7 allows laser capture microdissection. Virtually all steps are controlled for quality using Qubit Fluorometric Quantitation (Thermo Fisher Scientific), q-PCR, Bioanalyser (Agilent Genomics), LabChip (PerkinElmer), etc.

 

Our Sequencers

 

NXTGNT offers a wide range of sequencing apparatus allowing to sequence at the scale and scope of your project.

 

MiSeq

Illumina Miseq

The in-house MiSeq benchtop sequencer is well suited for smaller projects and thus allows for more focused applications such as targeted gene sequencing, metagenomics, small genome and transcriptome sequencing, and targeted gene expression. Generating 1 million up to 25 million reads, the MiSeq sequencer is ideal for reduced representation studies and narrowly focused applications.

 

MiSeq FGx Sequencing System

MiSeq FGx

The MiSeq FGx Sequencing System is the first and only next-generation sequencing (NGS) instrument developed and validated for forensic genomics. The first and only instrument to interrogate up to 96 combined SNP and STR libraries in a single run, the MiSeq FGx System preserves precious sample while demonstrating robust performance. Develop more thorough, detailed DNA profiles from a wide range of sample types, from high-quality genomic DNA (gDNA) to degraded, mixed, and limited samples.

 

NextSeq 500

Illumina NextSeq 500

With an output of up to 400 million reads, our in-house Illumina NextSeq 500 sequencer is more suited for larger study designs. The NextSeq 500 is easily configured providing researchers perfect scalability for mid-sized to large projects at maximal operational efficiency.

 

HiSeq 3000

Illumina HiSeq 3000

The HiSeq 3000 System is a powerful and efficient high-throughput sequencing system that, with an output of up to 750 gigabase (Gb) of data, is ideally suited for large-scale genomics projects.

Illumina Novaseq 6000

 

 

 

 

Novaseq 6000

 

The Novaseq 6000 System provides scalable ultra-high-throughput and flexibility for virtually any sequencing method, genome, and scale of project.

 

PromethION

PromethION 24

The Oxford Nanopore Technologies PromethION 24 is a single molecule, real-Time, large-scale, long-read, direct DNA and RNA sequencing sequencing system that provides high consensus accuracy and long read lengths, which makes it extremely suitable for whole-genome sequencing, de novo assemblies and metagenomics of viral populations. Moreover, nanopore sequencing can directly detect epigenetic modifications both on DNA and RNA, making it useful in epigenetics studies.

 

Address

NXTGNT
Ghent University,
Faculty of Pharmaceutical Sciences
Ottergemsesteenweg 460
B-9000 Gent
Belgium

 

Inside the building

When entering the Ghent University Faculty of Pharmaceutical Sciences through the main entrance, elevators will be located in front of you. NXTGNT is situated on the third floor. When exiting the elevators, NXTGNT is located at your right-hand side.

 

Contact us

nxtgnt@UGent.be

Call: +32 9 264 80 59