NXTGNT

About NXTGNT
Why work with us?

Core facility for next generation sequencing

(epi-)genome, transcriptome, ChIP

Proven solutions

Efficient, automated workflows

NXTGNT was founded in 2008 to create a rich and unique substrate for groundbreaking research, development and valorization at Ghent University. The main goal of NXTGNT is the establishment of a reference center and core facilty for next generation sequencing (NGS), (epi-)genome and transcriptome analysis at Ghent University. NXTGNT established a unique service platform by the integration of a state-of-the-art NGS library preparation facility with several sequencing platforms and a strong expertise in -omics data-analysis (bioinformatics).

 

Experience

Broad range of Applications
Innovative workflows
All organisms / species

Bioinformatics

From RNA / DNA to results

NXTGNT is supported by several Ghent University partners from different faculties:

Expertise and services

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Genomics

Using massively parallel sequencing the genome sequence can be accurately determined. Depending on your setting and interest the whole genome can be sequenced (even starting from a very limited amount of DNA) or specific genes or regions (e.g. exome sequencing) can be targeted.

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Transcriptomics

Transcriptomics (or expression profiling) is the study of the complete set of RNA transcripts that are produced by specific cells or tissues under specific circumstances. Messenger RNAs (mRNA), micro RNAs (miRNA), long non-coding RNAs (lncRNA), etc can be sequenced and quantified, allowing differential gene expression analysis.

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Epigenomics

Epigenetics research seeks to describe dynamic alterations in the transcriptional potential of a cell. The main epigenetic mechanisms are DNA methylation, histone modification, DNA accessibility and non-coding RNA-associated gene silencing. NXTGNT has all the tools in house to analyse these epigenetic mechanism in an integrated analysis.

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Bioinformatics

NXTGNT can help you with your sequencing data analysis with a team of high-skilled experts. We perform data analysis starting from raw sequencing reads, working towards publication-ready results: Statistics, visualization, PCA analysis, Gene-Set Enrichment Analysis, pathway analysis, etc. Feel free to contact us regarding any question.

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10X Genomics
single cell sequencing

We provide all single cell and linked-reads sequencing solutions offered by the 10X Genomics Chromium System & GemCode Technology

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Pharmacogenetics

Molecular insights, connecting physicians and their patients to the latest treatment approaches through pharmacogenetics, making precision medicine a reality.

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Nanopore sequencing

Oxford nanopore sequences unamplified DNA and RNA, eliminating the need for amplification steps (and the resulting quantitative and qualitative amplification bias). Long reads up to 100 kbp with direct detection of 5-methylcytosines in DNA and N6-methyladenines in RNA provide tremendous advantages in de novo assembly, detection of large structural variants, RNA isomforms, etc.

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Laser-capture microdissection (LCM)

Our PALM MicroBeam is designed to give you advanced laser microdissection capabilities for subsequent DNA, and RNA analysis — whether from archive material or live cells. Applicable to cryosections, and FFPE tissue, even from standard glass slides.

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Low input samples

NXTGNT is specialized in low input samples such as single cell samples, FFPE samples, and LCM samples

Meet the team

Filip Van Nieuwerburgh

Filip Van Nieuwerburgh

Steering Committee

Prof. Filip Van Nieuwerburgh
(Ph.D. Pharm.D.)

Filip Van Nieuwerburgh heads the UGent Next Generation Sequencing core facility NXTGNT, focusing on transcriptomics, (epi)genomics and bioinformatics. He coordinated the initiative to bring together key experts to create the Expertise Centre for Sequencing
Dieter Deforce

Dieter Deforce

Steering Committee

Prof. Dieter Deforce (Ph.D. Pharm.D.)

Dieter Deforce is the head of the Laboratory for Pharmaceutical Biotechnology in the Faculty of Pharmaceutical Sciences. He is the director of ProGenTomics offering proteomics and genomics services.
Wim Van Criekinge

Wim Van Criekinge

Steering Committee

Prof. Wim Van Criekinge (Ph.D.)

Wim Van Criekinge heads Biobix, a Computational Genomics & Bioinformatics laboratory at Ghent University. Wim Van Criekinge is CSO of MDxHealth, a listed biotechnology company active in cancer diagnostics.
Daisy Flamez

Daisy Flamez

Steering Committee

Daisy Flamez

Passionate towards innovation in applied biomarker research, business development and contract management, network and partnering with industrial partners, patent portfolio management, facilitation of obtaining (inter)national innovation funds, licensing, and setup of spin-offs.
Sarah De Keulenaer

Sarah De Keulenaer

Sequencing Operational Team

Sarah De Keulenaer

Project coordination. Sample preparation expert. Financial administration.
Sylvie Decraene

Sylvie Decraene

Sequencing Operational Team

Sylvie Decraene

Project coordination. Sample preparation expert. Financial administration.
Ellen De Meester

Ellen De Meester

Sequencing Operational Team

Ellen De Meester

Project coordination. Sample preparation expert. Financial administration.
Yannick Gansemans

Yannick Gansemans

Bioinformatics Team

Yannick Gansemans

A biologist, a chemist, and a statistician are out hunting. The biologist shoots at a deer and misses five feet to the left. The chemist takes a shot and misses five feet to the right. The statistician yells “We got ’em!”
Sonja Skevin

Sonja Skevin

Bioinformatics Team

Sonja Skevin

FASTA than the others
Laurentijn Tilleman

Laurentijn Tilleman

Bioinformatics Team

Laurentijn Tilleman

If you torture data enough it will confess.

Tim Meese

Bioinformatics Team

Tim Meese

Bring data! I’ll provide the results.
Biobix logo

Biobix

The BIOBIX lab of Bioinformatics and Computational Genomics

Biobix

The BIOBIX lab of Bioinformatics and Computational Genomics focuses the development of innovative data-analytical methodologies to solve complex biological questions, related to imprinting, CpG mutability, epitranscriptomics, telomere biology, micropeptide biology, cancer, aging studies, neuronal development, crop protection, virus discovery and ecology.

Contact

Address

NXTGNT
Ghent University,
Faculty of Pharmaceutical Sciences
Ottergemsesteenweg 460
B-9000 Gent
Belgium

 

Inside the building

When entering the Ghent University Faculty of Pharmaceutical Sciences through the main entrance, elevators will be located in front of you. NXTGNT is situated on the third floor. When exiting the elevators, NXTGNT is located at your right-hand side.

 

Contact us

nxtgnt@UGent.be

Call: +32 9 264 80 59