Whole Genome Amplification

If your amount of available DNA is too limited, it can be amplified before being sequenced by using Whole Genome Amplification (WGA). Currently, we routinely apply SurePlex DNA Amplification, DOPlify, REPLI-g and Ampli1 WGA, yielding 2 to 5 µg of DNA from input DNA as low as 1 cell (6 pg of DNA).


Advantages of Whole Genome Amplification

  • Low input amounts of DNA can be amplified to sufficient amounts for sequencing


Whole Genome Amplification workflow

For this small amount of sample, a quality control is not possible. We start directly with the DNA amplification followed by preparing the sequencing libraries. After a quality assessment step these libraries are sequenced on one of our Illumina sequencers. The technical quality of the sequencing run is monitored in real time.

Sequencing data can be transferred to you via the Illumina BaseSpace platform or our own server (FTP download).

Feel free to contact us with any questions.

Whole Genome Amplification


Ghent University,
Faculty of Pharmaceutical Sciences
Ottergemsesteenweg 460
B-9000 Gent


Inside the building

When entering the Ghent University Faculty of Pharmaceutical Sciences through the main entrance, elevators will be located in front of you. NXTGNT is situated on the third floor. When exiting the elevators, NXTGNT is located at your right-hand side.


Contact us


Call: +32 9 264 80 59